QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Add 300 µL of prewarmed serum free media to the interior of the inserts. Allow this to rehydrate the ECM layer for 15-30 minutes at room temperature.	Add 250 µL of prepared cell suspension from step 5 to each insert. Add 500 µL of serum free media in the presence or absence of chemoattractant. Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (46% CO2).

Upstream tips
Add 300 µL of prewarmed serum free media to the interior of the inserts. Allow this to rehydrate the ECM layer for 15-30 minutes at room temperature.
Protocol tips
Add 250 µL of prepared cell suspension from step 5 to each insert. Add 500 µL of serum free media in the presence or absence of chemoattractant. Cover plate and incubate for 24 - 72 hours at 37°C in a CO2 incubator (46% CO2).

Publication protocol

In vitro cell invasion assays were performed with the QCM ECMatrix 24-well (8 μM) Fluorimetric Cell Invasion Assay kit (Chemicon; Millipore) according to the manufacturer's instructions (17,18). The kit used an insert polycarbonate membrane with an 8 μM pore size. The insert in the invasion kit was coated with a thin layer of ECMatrix. Cell invasion was determined by fluorescence. Each experiment was repeated three times in duplicate

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Manufacturer protocol

Download the product protocol from Merck Millipore for QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), fluorimetric below.

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