Cultrex BME Cell Invasion Assay, 96 well

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	Incubate for 19 hours at 37ºC in 5% CO2 atmosphere

Protocol tips
Incubate for 19 hours at 37ºC in 5% CO2 atmosphere

Publication protocol

Cell invasion assays were carried out using Trevigen Cultrex 24 well cell invasion assay kits. Invasion chambers were coated with a 1× solution of Basement Membrane Extract (BME) per the manufacturer’s protocol and allowed to gel overnight. The following day, 5 × 104 serum starved HT1080 cells were added to the top of each chamber in 100 l of serum free media. Five hundred l of complete DMEM, which serves as the chemo-attractant, was added to the bottom of each well. Three chambers were treated with 200 nM CPE and three wells were left as untreated controls. After 19 h of incubation at 37°C, the chambers were removed and the assay was developed using a calcein marker included with the kit, and read on a fluorescent plate reader.

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