Oris™ Universal Cell Migration Assembly Kit, 96 wells

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Plate 15 000 cells per well	Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 8 hours (cell line dependent) to permit cell attachment.

Upstream tips
Plate 15 000 cells per well
Protocol tips
Incubate the seeded plate containing the Oris™ Cell Seeding Stoppers in a humidified chamber (37°C, 5% CO2) for 8 hours (cell line dependent) to permit cell attachment.

Publication protocol

The 96-well Oris Cell Migration Kit (CMAU101; Platypus Technologies, Fitchburg, WI, USA) was used for cell migration assays. Wells were coated with 8 μg/cm collagen I (C3867; Sigma, Gillingham, UK) for 2 h at room temperature. Following two washes in phosphate-buffered saline, silicone stoppers were inserted in the wells to create ‘exclusion zones'. Cells were plated in triplicate wells (15 000 cells per well) and incubated at 37 °C overnight. The medium was replaced with serum-free Dulbecco's modified Eagle's medium/2.5% bovine serum albumin and cells were incubated for 8 h at 37 °C before removing the silicone stoppers to start the assay by allowing cells to migrate into the exclusion zone. Cells were incubated for 24 h at 37 °C, fixed with 3.7% formaldehyde and stained with 0.05% crystal violet. Migration was quantified by taking low magnification images of each well, measuring the remaining area of the exclusion zone using Adobe Photoshop CS3 Extended and comparing it with the exclusion zone area in reference wells (no migration).

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Manufacturer protocol

Download the product protocol from Platypus Technologies for Oris™ Universal Cell Migration Assembly Kit, 96 wells below.

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