24-Well Cell Invasion Assays, Basement Membrane

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seed 300,000 cells/well	Incubate for 48 hours at 37ºC in 5% CO2 atmosphere.

Upstream tips
Seed 300,000 cells/well
Protocol tips
Incubate for 48 hours at 37ºC in 5% CO2 atmosphere.

Publication protocol

The invasive potential of the investigated cells was evaluated using the colorimetric CytoSelect™ 24-well invasion assay (Cell Biolabs, Inc.). The assay contains a membrane coated with a layer of basement membrane matrix solution and allows for discrimination of invasive cells. The cells were seeded on inserts placed in a 24-well plate at a density of 300,000 cells/well and left to invade for 48 h. Next, the cells that crossed the membrane were dyed and their number was analyzed colorimetrically.

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