Cultrex® BME Cell Invasion Assay

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Coat with 100 µl basement membrane extract coating solution at 37°C for 4 h.	Incubate at 37C in a CO2 incubator for 24 hours

Upstream tips
Coat with 100 µl basement membrane extract coating solution at 37°C for 4 h.
Protocol tips
Incubate at 37C in a CO2 incubator for 24 hours

Publication protocol

The invasion assay was carried out following the instructions of Cultrex 24-well Transwell BME cell invasion assay (Trevigen). Briefly, a 24-well unit with 8 µm polycarbonate nucleopore filters (Corning) evenly coated with 100 µl basement membrane extract coating solution (Trevigen) at 37°C for 4 h. Control A549 cells (2×105) expressing GFP and RAW 264.7 macrophages (1×105) in serum free medium were placed in the upper compartment, and 50% RPMI 1640 plus 50% DMEM medium supplied with 0.5% FBS was added to the lower compartment. After 24 h incubation, cells that had not invaded were removed with a cotton swab. Cells that had invaded the lower surface of the membrane were observed under a fluorescence microscope. Invaded cells in three randomly selected fields were counted.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell migration / Invasion cell type - RAW 264.7 using Cultrex® BME Cell Invasion Assay from Bio-Techne.

Manufacturer protocol

Download the product protocol from Bio-Techne for Cultrex® BME Cell Invasion Assay below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell migration / Invasion cell type - RAW 264.7 using Cultrex® BME Cell Invasion Assay from Bio-Techne. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.