CultreCoat Low BME Cell Invasion Assay, 96 well

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate at 37 oC in a CO2 incubator for 24 hours. On the third day add 100 µl of Cell Dissociation Solution/ Calcein AM to bottom chamber and incubate at 37 oC in a CO2 incubator for one hour	Read plate at 485 nm excitation, 520 nm emission.

Protocol tips
Incubate at 37 oC in a CO2 incubator for 24 hours. On the third day add 100 µl of Cell Dissociation Solution/ Calcein AM to bottom chamber and incubate at 37 oC in a CO2 incubator for one hour
Downstream tips
Read plate at 485 nm excitation, 520 nm emission.

Publication protocol

CultreCoat® Low BME Cell Invasion Assay was purchased from R&D Systems. Cells were plated in 96 well CultreCoat® plates with either vehicle or R428. 24 hours post therapy cell invasion was measured as per manufactures instructions.

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Manufacturer protocol

Download the product protocol from R&D Systems for CultreCoat Low BME Cell Invasion Assay, 96 well below.

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