EZCell™ Cell Invasion Assay (Basement Membrane), 24-well, 8 µm

Protocol tips

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	Cover and incubate the Cell Migration Chamber at 37°C in CO2 incubator for 24-48 hrs

Protocol tips
Cover and incubate the Cell Migration Chamber at 37°C in CO2 incubator for 24-48 hrs

Publication protocol

MM cell invasion was measured using an 8-μ-pore transwell plate coated with gel basement membrane solution (BioVision, Inc., USA) according to the manufacturer’s protocols. Next, 2–3 × 105 serum starved MM cells (LP1 and RPMI-8226), control or ITAG8 overexpressed, suspended in serum free RPMI1640 media were added to the upper chamber in the presence of supplemented medium in the lower chamber. After 24–48 h of incubation, the cells in the lower chamber were counted using a Countess II FL (Thermo Fisher Scientific, USA) or the O.D. value was measured at 450 nm.

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