Endothelial Tube Formation Assay (In Vitro Angiogenesis)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- 200 µL of thawed ECM gel solution to each well. - 600 uL of cell suspension was added onto the solidified ECM gel and incubated the assay plate at 37ºC for18 hrs.

Protocol tips
- 200 µL of thawed ECM gel solution to each well. - 600 uL of cell suspension was added onto the solidified ECM gel and incubated the assay plate at 37ºC for18 hrs.

Publication protocol

Angiogenesis in vitro was performed utilizing the endothelial tube formation assay kit (Cell Biolabs, CA), according to the instruction. The extracellular matrix gel was prepared from Engelbreth-Holm-Swarm tumor cells (200 μl/ well). HUVECs (ten thousand cells/well) infected with retroviruses or cultured with CM from PANC-1 cells that were infected with the indicated retroviruses. The mixture was added onto solidified extracellular matrix gel in 600 μl medium. After incubation for 18 h, endothelial cell tube formation was assessed and tube number was counted.


Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Angiogenesis assay human - HUVEC using Endothelial Tube Formation Assay (In Vitro Angiogenesis) from Cell Biolabs.

Manufacturer protocol

Download the product protocol from Cell Biolabs for Endothelial Tube Formation Assay (In Vitro Angiogenesis) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Angiogenesis assay human - HUVEC using Endothelial Tube Formation Assay (In Vitro Angiogenesis) from Cell Biolabs. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.