In Vitro Angiogenesis Assay Kit

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	Cells are observed by microscopy after 6h of incubation.

Protocol tips
Cells are observed by microscopy after 6h of incubation.

Publication protocol

Capillary tube formation was used to assess the angiogenic potential of releasates generated from resting platelets or platelets stimulated with agonists using the Millipore Capillary Tube Formation Assay kit (Billerica, MA) in duplicate. Primary human umbilical vein endothelial cells (HUVECs) were purchased from Lonza (Walkersville, MD) and cultured in EBM media (Lonza) according to their protocol. After a 6-hour incubation of HUVEC cells with platelet releasate, capillary tube formation was quantified. Five fields were imaged (at ×4 and ×20 magnification) with differential-interference-contrast microscopy using a Zeiss Axiovert microscope, and the degree of tubulogenesis was quantified by counting branch points (nodes with 3 or more branches). Independent assays were averaged, and statistical analysis was performed using the Student t test.


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