Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit

Angiogenesis assay human - HMVEC (human microvascular lung endothelial cells)

Experiment
Angiogenesis assay human - HMVEC (human microvascular lung endothelial cells)
Product
Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit from Trevigen
Manufacturer
Trevigen

Protocol tips

Protocol tips
- After seeding for 24h cells are treated with Galectin-3 for another 24h.

Publication protocol

HMVECs (1×105 cells/well) were cultured in 24-well plates for 24 hr before treatment with galectin-3 for 24 hr. The culture media was collected and used, with or without subsequent introduction of a combination of recombinant cytokines or a combination of neutralizing anti-cytokine antibodies, to assess migration of fresh HMVECs through basement matrix proteins using the In Vitro Angiogenesis Assay Endothelial Cell Invasion kit; or to assess HUVECs tubule formation using the In Vitro Angiogenesis Assay Endothelial Cell Tube Formation kit. The length and branch points of tubules formed were quantified using ImageJ (http://rsbweb.nih.gov/ij/).


Full paper   Login or join for free to view the full paper.

Reviews

Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit from Trevigen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

2 years ago

Author: Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Angiogenesis assay human - HMVEC (human microvascular lung endothelial cells) using Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit from Trevigen.

Paper title
Increased circulation of galectin-3 in cancer induces secretion of metastasis-promoting cytokines from blood vascular endothelium
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Trevigen for Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Angiogenesis assay human - HMVEC (human microvascular lung endothelial cells) using Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit from Trevigen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms