Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit

Protocol tips

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	- After seeding for 24h cells are treated with Galectin-3 for another 24h.

Protocol tips
- After seeding for 24h cells are treated with Galectin-3 for another 24h.

Publication protocol

HMVECs (1×105 cells/well) were cultured in 24-well plates for 24 hr before treatment with galectin-3 for 24 hr. The culture media was collected and used, with or without subsequent introduction of a combination of recombinant cytokines or a combination of neutralizing anti-cytokine antibodies, to assess migration of fresh HMVECs through basement matrix proteins using the In Vitro Angiogenesis Assay Endothelial Cell Invasion kit; or to assess HUVECs tubule formation using the In Vitro Angiogenesis Assay Endothelial Cell Tube Formation kit. The length and branch points of tubules formed were quantified using ImageJ (http://rsbweb.nih.gov/ij/).


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