Corning® BioCoat™ Angiogenesis System: Endothelial Cell Tube Formation

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- The pretreated cells plus RA SF (1:20) or flagellin (10 ng/ml) were added to the polymerized matrigel and the plate was incubated for 16h at 37°C

Protocol tips
- The pretreated cells plus RA SF (1:20) or flagellin (10 ng/ml) were added to the polymerized matrigel and the plate was incubated for 16h at 37°C

Publication protocol

To perform the matrigel tube formation assay, BD Matrigel Matrix (BD BioCoat tube formation kit, 50 µl) was polymerized for 30 min at 37°C in a 96-well plate. In order to determine whether TLR5 ligation is pivotal for RA SF induced endothelial tube formation, HMVECs/HUVECs were incubated with antibody to TLR5 or IgG (10µg/ml) for 45 minutes at 37°C. The pretreated cells plus RA SF (1:20) or flagellin (10 ng/ml) were added to the polymerized matrigel and the plate was incubated for 16h at 37°C. Each condition was performed in triplicate, bFGF (20 ng/ml; R&D systems) was used as a positive control, and PBS was used as a negative control. Following incubation, the number of branch points/tubes was quantified as previously described (17, 19, 20).


Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Angiogenesis assay human - HMVEC using Corning® BioCoat™ Angiogenesis System: Endothelial Cell Tube Formation from Corning.

Manufacturer protocol

Download the product protocol from Corning for Corning® BioCoat™ Angiogenesis System: Endothelial Cell Tube Formation below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Angiogenesis assay human - HMVEC using Corning® BioCoat™ Angiogenesis System: Endothelial Cell Tube Formation from Corning. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.