In Vitro Angiogenesis Assay Kit

Angiogenesis assay human - Ea.Hy926

Experiment
Angiogenesis assay human - Ea.Hy926
Product
In Vitro Angiogenesis Assay Kit from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
- Cells are seeded with Endothelial Cell Basal Media (EBM-2). Cells are observed microscopically and photographed after 17–24 h incubation.

Publication protocol

Endothelial tube formation was measured utilizing the in vitro Angiogenesis Assay Kit (Millipore, Billerica, MA) as we have previously described [32]. EA.hy926 (1x104) cells were resuspended in EBM-2 (Lonza Group, Walkersville, MD) medium and plated onto the polymerized extracellular matrix. The formation of endothelial tubes was observed microscopically and photographed after 17–24 h incubation. Tube formation was quantified by branch point counting as specified by the manufacturer.

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Discussion

Discussion

5 years ago

Author: Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Angiogenesis assay human - Ea.Hy926 using In Vitro Angiogenesis Assay Kit from Merck Millipore.

Paper title
Exosome-mediated microRNA signaling from breast cancer cells is altered by the anti-angiogenesis agent docosahexaenoic acid (DHA)
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Manufacturer protocol

Download the product protocol from Merck Millipore for In Vitro Angiogenesis Assay Kit below.

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