In Vitro Angiogenesis Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Incubate at 37C for at 2 hours to allow the matrix solution to solidify.

Protocol tips
- Incubate at 37C for at 2 hours to allow the matrix solution to solidify.

Publication protocol

Matrigel assay was used to evaluate capillary tube formation activity as described in the protocol of In Vitro Angiogenesis Assay Kit (Millipore Co., USA). Briefly, ECMatrix™ solution was thawed on ice overnight, then mixed with diluent and placed in a 96-well plate at 37° for 2h to allow the matrix solution to solidify. Each group ECV304 was added on the polymerized matrigel at 104 cells per well. After incubation at 37° for 10h, capillary tubes were inspected at a magnification of 400× with an inverted phase contrast microscope. Capillary tubes were defined as endothelial cord formations that were connected at both ends. The number of tubes in six random fields per well was determined


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Manufacturer protocol

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