In Vitro Angiogenesis Assay Kit

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	- Cells (80,000) mixed with different concentration of complex 1 (0, 6, 12, 24 µM) in 100 µL DMEM medium containing Endothelial Cell Growth Supplements (ECGS) (0.05 µg/ µL)

Protocol tips
- Cells (80,000) mixed with different concentration of complex 1 (0, 6, 12, 24 µM) in 100 µL DMEM medium containing Endothelial Cell Growth Supplements (ECGS) (0.05 µg/ µL)

Publication protocol

In Vitro Angiogenesis Kit (Millipore Corporation). 10× Diluent Buffer was mixed with ECMatrixTM solution in the ratio of 1:9, and 50 µL of the mixture was transferred to each well of 96 well plates which was further incubated at 310 K for 1 h allowing polymerization. Then, MS1 cells (80,000) mixed with different concentration of complex 1 (0, 6, 12, 24 µM) in 100 µL DMEM medium containing Endothelial Cell Growth Supplements (ECGS) (0.05 µg/ µL) was added on the top of the polymerized matrix. After incubated at 310 K for 2 h, tube formation was investigated with a Zeiss Axiovert 200M inverted fluorescence microscopy at a 5× magnification


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