In Vitro Angiogenesis Assay Kit

Protocol tips

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	- Supplemented with 1% fetal bovine serum and with or without 50 ng/ml vascular endothelial growth factor (VEGF) as chemo-attractant and incubated at 37°C for 24 hours.

Protocol tips
- Supplemented with 1% fetal bovine serum and with or without 50 ng/ml vascular endothelial growth factor (VEGF) as chemo-attractant and incubated at 37°C for 24 hours.

Publication protocol

Tube formation of the hiPSC-1-EC, hiPSC-2-EC, hESC-EC, BM-EC and HUVEC were assessed with the In-Vitro Angiogenesis Assay Kit (Chemicon, Temecula, CA) with 1×104 cells as described with modifications [9]. Modified Boyden Chamber assay was preformed with 1×104 cells of cells (hiPSC-1-EC, hiPSC-2-EC, hESC-EC, BM-EC and HUVEC) placed in the upper chamber of the Transwell® pore Polycarbonate Membrane Insert (Corning, Lowell, MA) in EBM2 medium with 1% fetal bovine serum. The chamber was placed in a 24-well plate containing EGM2 medium with or without 50 ng/ml vascular endothelial growth factor (VEGF) as chemo-attractant and incubated at 37°C for 24 hours. Then the lower side of the filter was washed with phosphate buffered saline and fixed with 2% paraformaldehyde. Migrated cells were visualized by hematoxylin staining.


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Manufacturer protocol

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