Phusion Site-Directed Mutagenesis Kit

Site Directed Mutagenesis (SDM) Rat - Deletion H9C2 SRF

Experiment
Site Directed Mutagenesis (SDM) Rat - Deletion H9C2 SRF
Product
Phusion Site-Directed Mutagenesis Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
Phusion Hot Start II DNA Polymerase tends to work better at elevated denaturation and annealing temperatures due to higher salt concentrations in its buffer. Hence follow manufacturer instruction strictly.

Publication protocol

2.10 Mutagenesis of 3′UTRs
Deletion of the miR-1 seed sequence was carried out using Phusion Site-directed Mutagenesis Kit (Finnzymes).



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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Site Directed Mutagenesis (SDM) Rat - Deletion H9C2 SRF using Phusion Site-Directed Mutagenesis Kit from Thermo Fisher Scientific.

Paper title
An SRF/miR-1 axis regulates NCX1 and annexin A5 protein levels in the normal and failing heart.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Phusion Site-Directed Mutagenesis Kit below.

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