GeneBLAzer In Vivo Detection Kit
Reporter gene assay β-lactamase substrates - HeLa
Publication protocol
The presence of fusion proteins in the cytosol of infected HeLa cells was observed directly with the use of the GeneBLAzer In Vivo Detection Kit (Invitrogen). HeLa monolayers were cultivated on glass cover slips to a confluence of ca. 75% and infected with . L2 expressing various β-lactamase-fusion proteins. CCF2-AM substrate was applied 24 hpi for 30 minutes, samples were fixed in 4% paraformaldehyde, and fluorescence was observed using a Leica TCS SP5 laser scanning confocal microscope. Images were processed equivalently using Adobe Photoshop CS2 version 9.0 (Adobe Systems, San Jose, CA).Full paper Login or join for free to view the full paper.
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