Muse® Cell Cycle Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- If low level of staining is noticed, increase volume of stain used.

Protocol tips
- If low level of staining is noticed, increase volume of stain used.

Publication protocol

Cells were harvested at least 3 hours before the experiments. After fixation with 1ml of 70% cold ethanol at -20°C, as indicated by the Muse Cell Cycle Kit User’s Guide, 200μl of ethanol-fixed cells were incubated with PI and RNAse A for 30 minutes at room temperature, before loading on Muse Cell Analyzer (Millipore, Italy) according to the supplied staining protocol.


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Manufacturer protocol

Download the product protocol from Merck Millipore for Muse® Cell Cycle Assay Kit below.

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