Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Cells were labelled with 10 μmol/L of 5-ethynyl-2′-deoxyuridine and incubated for 60 minutes at 37°C. - Cells were fixed with 4% paraformaldehyde for 60 minutes instead of 15min. - 7-AAD was added for measuring DNA content and cell cycle distribution.

Protocol tips
- Cells were labelled with 10 μmol/L of 5-ethynyl-2′-deoxyuridine and incubated for 60 minutes at 37°C. - Cells were fixed with 4% paraformaldehyde for 60 minutes instead of 15min. - 7-AAD was added for measuring DNA content and cell cycle distribution.

Publication protocol

Cell cycle analysis was performed using a Click-iT EdU Pacific Blue flow cytometry assay kit according to the manufacturer's protocol. In brief, 10 μmol/L of 5-ethynyl-2′-deoxyuridine (EdU) was added into culture medium, and cells were incubated for 60 minutes at 37°C. Cells were fixed with 4% paraformaldehyde for 60 minutes, and then the EdU was labeled with Pacific Blue. 7-Aminoactinomycin D (7-AAD) was added for measuring DNA content and cell cycle distribution, and cell cycle analysis was performed using a BD FACSAria II flow cytometer.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit below.

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