BD Cycletest™ Plus DNA Kit

Protocol tips

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- Cells were seeded andstarved overnight to achieve synchronization and then treated with 0–200 μM of H2O2 for 24 h.

Upstream tips
- Cells were seeded andstarved overnight to achieve synchronization and then treated with 0–200 μM of H2O2 for 24 h.

Publication protocol

The cell cycle and apoptosis were analyzed by flow cytometry (FCM). To assess the cell cycle, HepG2 cells in logarithmic phase were harvested and plated in 24-well culture plates at a concentration of 2 ∗ 105 cells/mL. Cells were starved overnight to achieve synchronization and then treated with 0–200 μM of H2O2 for 24 h. Using FCM instrument (FACS Calibur, BD Biosciences, San Jose, CA, USA), cell cycle was detected with cycle test Plus DNA reagent kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer's instructions.

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