BD Cycletest™ Plus DNA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells were seeded and treated with vehicle control, 100nM free paclitaxel, 15ug/mL α-CEA-PTX, or 15ug/mL α-CEA-680-PTX (both at 100nM effective paclitaxel) for 24 hours.

Upstream tips
- Cells were seeded and treated with vehicle control, 100nM free paclitaxel, 15ug/mL α-CEA-PTX, or 15ug/mL α-CEA-680-PTX (both at 100nM effective paclitaxel) for 24 hours.

Publication protocol

Cell cycle analysis was performed using the Becton Dickson BD Cycletest™ Plus DNA Reagent Kit (BD, #340242, San Jose, CA). BxPC-3 cells were seeded equally into T25 flasks, and allowed to recover for 24 hours. Flasks were then treated with vehicle control, 100nM free paclitaxel, 15ug/mL α-CEA-PTX, or 15ug/mL α-CEA-680-PTX (both at 100nM effective paclitaxel) for 24 hours. Cells were then harvested with Trypsin-EDTA, stained according to the kit instructions, and analyzed via flow cytometry as previously detailed. All samples were gated and analyzed identically per the manufacturer/template instructions, with the cell cycle histograms containing approximately 30,000–40,000 events.

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Manufacturer protocol

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