BD Cycletest™ Plus DNA Kit

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- cells were adjusted to a concentration of 1×106 cells/ml

Upstream tips
- cells were adjusted to a concentration of 1×106 cells/ml

Publication protocol

Cell cycle status was determined with a FACSCalibur flow cytometer (BD Biosciences). Following trypsinization, cells were adjusted to a concentration of 1×106 cells/ml and treated using Cycletest Plus DNA reagent kit (BD Biosciences), according to the manufacturer's instructions. The cell cycle status was analyzed by flow cytometry using propidium iodide (PI).

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Manufacturer protocol

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