Guava Cell Cycle Reagent for Flow Cytometry

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cells weretransfected with an inhibitor for 48h.	- Cells were stained for 20 min using Guava Cell Cycle Reagent.

Upstream tips
- Cells weretransfected with an inhibitor for 48h.
Protocol tips
- Cells were stained for 20 min using Guava Cell Cycle Reagent.

Publication protocol

Cells (5Y4b or 5Y) transfected for 48 h with inhibitor were harvested and then stained with 200 μl for 20 min as Guava Cell Cycle Reagent Kit (Millipore) instructions. Analysis of cell cycle analysis was performed according to the manufacturer’s protocol. Flow cytometry analysis was performed using the Beckman Coulter flow cytometer and EXPO32 software (Beckman).

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Manufacturer protocol

Download the product protocol from Merck Millipore for Guava Cell Cycle Reagent for Flow Cytometry below.

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