Muse® Cell Cycle Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
It is important to have a single cell suspension prior to ethanol fixation	Resuspend the cell pellet in 200 μL of Muse™ Cell Cycle Reagent.

Upstream tips
It is important to have a single cell suspension prior to ethanol fixation
Protocol tips
Resuspend the cell pellet in 200 μL of Muse™ Cell Cycle Reagent.

Publication protocol

Direct cell counting was performed after Nigrosin Blue (0.1% (w/v)) staining using a haemocytometer and light
microscopy; cells which excluded the dye were considered to be viable. MTS assays were performed according to the manufacturer’s instructions. For clonogenic assays, the colonies formed following 3 weeks of culture were counted after staining withCrystal Violet (0.5% (w/v) in methanol).Cell cycle analysis was performed on a Muse Cell Analyzer using a Muse Cell Cycle Assay Kit according to the supplied instructions."


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Manufacturer protocol

Download the product protocol from Merck Millipore for Muse® Cell Cycle Assay Kit below.

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