Guava Cell Cycle Reagent for Flow Cytometry

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cels were seeded for 24 hours, and incubated in the absence or presence of erucin for an another 24 hours,

Upstream tips
- Cels were seeded for 24 hours, and incubated in the absence or presence of erucin for an another 24 hours,

Publication protocol

Cell cycle analysis was performed using a Guava EasyCyte flow cytometer (Guava Technologies, Inc., Hayward CA) equipped with Cytosoft software 2.0. Briefly, non-synchronized MCF7 cells were seeded at 6×104 cells in 2 ml for 24 hours, and incubated in the absence or presence of erucin for an additional 24 hours, harvested, fixed in cold 70% ethanol and stained using Guava Cell Cycle Reagent (Guava Technologies, Inc., Hayward CA) according to the manufacturer’s protocol. The DNA content of at least 5000 cells was measured for each condition. Cell cycle distribution was analyzed by ModFit LT 3.1 software (Verity Software House, Inc., Topsham, ME). Results are the mean and SEM of at least three independent experiments.

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