Cell Cycle and Apoptosis Analysis Kit

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		- The red fluorescence was detected by a flow cytometer at an excitation wavelength of 488 nm.

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- The red fluorescence was detected by a flow cytometer at an excitation wavelength of 488 nm.

Publication protocol

In Vitro Cell Growth Assay
The SKOV3 cells were divided into the following 4 groups: cells transfected with PIRES2-EGFP-ARHI plasmid (treated group), cells transfected with PIRES2-EGFP plasmid (positive control group), cells without transfection (negative control group), and a blank control without cells. Cells were passaged at the logarithmic growth phase, trypsinized, and adjusted to 5 × 10 cells/mL. The cells were then seeded in 96-well plates in 100 µL 10% fetal bovine serum and cultured for 24, 48, 72, 96, and 120 hours at 37°C in a humidified 5% CO atmosphere. Each experimental group included 6 wells, and all experiments were repeated in triplicate. After incubation for 4 hours under the same condition, 10 µL CCK-8 reagent was added to each well. The optical density value of each well was measured using a microculture plate reader at a wavelength of 450 nm. The growth inhibitory rate (IR) was calculated using the following formula:

Apoptosis Assay
The SKOV3 cells were divided into the following 3 groups: PIRES2-EGFP-ARH–transfected group (treated), PIRES2-EGFP–transfected group (positive control), and cells without transfection (negative control). Each experimental group included 4 wells. The cells were harvested after 48 and 72 hours, and cell apoptosis was detected using Cell Cycle and Apoptosis Analysis Kits (Haimen, China), following the manufacturer’s instructions.

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