Muse® Cell Cycle Assay Kit

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	- Cells were treated with DMSO (vehicle) or various concentrations of carnosol.

Protocol tips
- Cells were treated with DMSO (vehicle) or various concentrations of carnosol.

Publication protocol

The cell cycle distribution analysis in DMSO- or carnosol-treated MDA-MB-231 cells was performed with the Muse Cell Analyzer (Millipore, Hayward, CA, USA) using the Muse cell cycle kit (Millipore, Hayward, CA, USA) according to the manufacturer's instructions. Briefly, cells grown onto 6 cm culture dish were treated with DMSO (vehicle) or various concentrations of carnosol. After 24 h of treatment, cells were collected by trypsinization, washed in PBS and resuspended in complete media and the Muse cell cycle test reagent was then added to each test tube. Cells were then incubated for 30 min at room temperature in the dark. After staining, the cells were processed for cell cycle analysis. Percentage of cells in G0/G1, S and G2/M phases were determined using the FlowJo software.

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