Guava Cell Cycle Reagent for Flow Cytometry

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Post seeding, cells were treated with various doses of DOX or PTX with or without 5 μM of STFD for 48 hr.	- Cells were fixed in 95% of ethanol overnight and stained

Upstream tips
- Post seeding, cells were treated with various doses of DOX or PTX with or without 5 μM of STFD for 48 hr.
Protocol tips
- Cells were fixed in 95% of ethanol overnight and stained

Publication protocol

K562/Adr cells were plated and incubated for 24 hr. The cells were treated with various doses of DOX or PTX with or without 5 μM of STFD for 48 hr. The cells were collected and fixed in 95% of ethanol overnight and stained with Guava® Cell Cycle reagent. The cell cycle phase distributions were determined on a Guava® PCA Instrument using Guava® easyCyte HT Software. In each experiment, determinations were carried out in triplicate.

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