PI/RNASE Solution

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		- After cells were treated with PI/RNase staining solution (Immunostep),they were incubated for 20 minutes at RT

Downstream tips
- After cells were treated with PI/RNase staining solution (Immunostep),they were incubated for 20 minutes at RT

Publication protocol

Cells were washed in PBS and fixed in 70% ethanol for later use. Cells were rehydrated with PBS, resuspended in 500 μl of PI/RNase staining solution (Immunostep), and incubated for 20 minutes at RT in the dark. Samples were analyzed using a FACSCalibur flow cytometer.

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