Guava Cell Cycle Reagent for Flow Cytometry

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Cells were harvested, fixed and stained with Reagent on day 0, 1, 3, 5 and 7 after seeding

Protocol tips
- Cells were harvested, fixed and stained with Reagent on day 0, 1, 3, 5 and 7 after seeding

Publication protocol

Control and SAMHD1 KO THP-1 cells were seeded, in triplicate, in 12-well plates at a density of 2×105 cells per well. At day 0, 1, 3, 5 and 7 after seeding, cells were counted, harvested, fixed in 70% ice-cold ethanol, and stained with Guava Cell Cycle Reagent (EMD Millipore), according to the manufacturer’s instructions. Cell cycle data was then acquired on the Guava flow cytometer instrument and analyzed using Cytosoft 4.2.

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Manufacturer protocol

Download the product protocol from Merck Millipore for Guava Cell Cycle Reagent for Flow Cytometry below.

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