APC BrdU Flow Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Dilute an appropriate amount of the antibody stock solution 1:50 with 1X BD Perm/Wash Buffer - Store unused portions of 1X BD Perm/Wash Buffer at 4°C.

Upstream tips
- Dilute an appropriate amount of the antibody stock solution 1:50 with 1X BD Perm/Wash Buffer - Store unused portions of 1X BD Perm/Wash Buffer at 4°C.

Publication protocol

Cells (2×104/100 μL) were plated in 96-well plates and incubated with different concentrations of FK866 or AC 93253 for the indicated times. We used the CellTiter-Glo Luminescence Cell Proliferation Assay to assess cell proliferation. To analyze apoptosis we used the Caspase-Glo 3/7 Activity Assay and counted viable cells using trypan blue dye exclusion. Both assays were performed according to the manufacturer’s instructions (Promega). For cell cycle analysis we measured BrdU uptake (20 min) using a BrdU Flow Kit (Pharmingen).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cycle assay human - HL-60 using APC BrdU Flow Kit from BD Biosciences.

Manufacturer protocol

Download the product protocol from BD Biosciences for APC BrdU Flow Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cycle assay human - HL-60 using APC BrdU Flow Kit from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.