Muse® Cell Cycle Assay Kit

Protocol tips

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	- Following seeing, cells were treated with PKF118-310, caperatic acid, physodic acid or camptothecin. - At −20 °C for at least 24 h cell are fixed with 70% ethanol.

Protocol tips
- Following seeing, cells were treated with PKF118-310, caperatic acid, physodic acid or camptothecin. - At −20 °C for at least 24 h cell are fixed with 70% ethanol.

Publication protocol

The analysis of cell cycle was performed using Muse Cell Cycle Kit (Merck, Germany) according to the manufacturer’s protocol. Briefly, cells were grown for the indicated time in the presence of the tested compounds (PKF118-310, caperatic acid, physodic acid) or camptothecin (a positive control) and upon termination of incubation cells were fixed in 70% ethanol and stored at −20 °C for at least 24 h. Subsequently, cells were washed with PBS buffer and stained with propidium iodide in the presence of RNase A. Next, samples were analyzed by flow cytometry on Muse Cell Analyzer and data were analyzed using Muse 1.5 Analysis software. All the experiments were repeated twice.

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Manufacturer protocol

Download the product protocol from Merck Millipore for Muse® Cell Cycle Assay Kit below.

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