PI/RNase Staining Buffer

Protocol tips

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- Cells are seeded and treated with UV-C and incubated with/without DMEM 10 μM of cisplatin for 96 h.

Upstream tips
- Cells are seeded and treated with UV-C and incubated with/without DMEM 10 μM of cisplatin for 96 h.

Publication protocol

Cell cycle analysis was done as described previously [7]. In brief, SW480 cells were exposed to UV-C, followed by the incubation in DMEM with/without 10 μM of cisplatin for 96 h. The cells were then harvested and stained with 500 μl of PI/RNase staining buffer for 15 min at room temperature. They were finally analyzed by flow cytometry using a FACS Calibur instrument (Becton Dickinson); data were analyzed using the CELL Quest computer program (Becton Dickinson) as previously described. All data were obtained from at least three independent experiments.

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