PI/RNase Staining Buffer

Cell cycle assay human - SW480

Experiment
Cell cycle assay human - SW480
Product
PI/RNase Staining Buffer from BD Biosciences
Manufacturer
BD Biosciences

Protocol tips

Upstream tips
- Cells are seeded and treated with UV-C and incubated with/without DMEM 10 μM of cisplatin for 96 h.

Publication protocol

Cell cycle analysis was done as described previously [7]. In brief, SW480 cells were exposed to UV-C, followed by the incubation in DMEM with/without 10 μM of cisplatin for 96 h. The cells were then harvested and stained with 500 μl of PI/RNase staining buffer for 15 min at room temperature. They were finally analyzed by flow cytometry using a FACS Calibur instrument (Becton Dickinson); data were analyzed using the CELL Quest computer program (Becton Dickinson) as previously described. All data were obtained from at least three independent experiments.

Full paper   Login or join for free to view the full paper.

Reviews

PI/RNase Staining Buffer from BD Biosciences has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Cell cycle assay human - SW480 using PI/RNase Staining Buffer from BD Biosciences.

Paper title
Cisplatin and ultra-violet-C synergistically down-regulate receptor tyrosine kinases in human colorectal cancer cells
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from BD Biosciences for PI/RNase Staining Buffer below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Cell cycle assay human - SW480 using PI/RNase Staining Buffer from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms