APC BrdU Flow Kit

Protocol tips

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	Cells are treated with 0.2 μM TPT, 0.5 μM PF477736, or both incubated for 16 hours. Cells were incubated for 30min after adding BrdU.

Protocol tips
Cells are treated with 0.2 μM TPT, 0.5 μM PF477736, or both incubated for 16 hours. Cells were incubated for 30min after adding BrdU.

Publication protocol

Cells were seeded at 8 X 105/60 mm plate and 8 hours prior to drug treatment. Fresh complete medium containing 0.2 μM TPT, 0.5 μM PF477736, or both drugs was added and cells were incubated for 16 hours. The procedure was performed according to manufacturer’s protocol (BD Pharmingen APC BrdU Flow kit). Following 30 minutes incubation after adding BrudU, cells were trypsinized and collected for subsequent fixation and permeabilization. Cells were analyzed by FACS Calibur (Becton Dickinson) after staining with APC-anti-BrdU and 7-AAD. Cell cycle was analyzed using FlowJo software.

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Manufacturer protocol

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