Muse® Cell Cycle Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		When the cell count is less, ensure enough amount of cell were seeded at the start of the experiment

Downstream tips
When the cell count is less, ensure enough amount of cell were seeded at the start of the experiment

Publication protocol

Cell cycle analysis was carried out using the Muse Cell Cycle Kit and Muse Cell Analyzer (Merck Millipore, Darmstadt, Germany). Cells were harvested by trypsinization and fixed by 70% ice-cold ethanol. After fixation, cell pellets were resuspended in Muse Cell Cycle Kit reagent, incubated for 30 min, and protected from light until further analysis. Cell cycle analysis carried out by the method supplied by the manufacturer

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Manufacturer protocol

Download the product protocol from Merck Millipore for Muse® Cell Cycle Assay Kit below.

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