FxCycle™ PI/RNase Staining Solution

Protocol tips

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	- Cells were fixed at 4 °C for 1 h and stored at −20 °C until ready

Protocol tips
- Cells were fixed at 4 °C for 1 h and stored at −20 °C until ready

Publication protocol

Cells were fixed with 70% ethanol. Briefly, cells were collected and washed in PBS–2%BSA twice and resuspended to a concentration of 3–6 mill/ml. 500 ml were taken and 5 ml cold 70% ethanol was added dropwise to the cells whilst gently vortexing to prevent cell clumping. Cells were fixed at 4 °C for 1 h and stored at −20 °C until ready for FACS analysis. The cells were stained with FxCycle PI/RNase Staining Solution solution (Life Technologies F10797) according to the manufacturer’s protocol and the cells were analysed using the BD FACS Canto 11 system with the BD FACS Diva software version 8 (BD Biosciences, San Jose, CA, USA). Data analysis was performed using the Modfit4 software (Verity Software House, Topsham, ME, USA).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for FxCycle™ PI/RNase Staining Solution below.

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