PI/RNase Staining Buffer

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- cells were fixed in ice-cold 70% ethanol at 4°C for >12h. - After staining cells were incubate 15 minutes instead of 10min.

Protocol tips
- cells were fixed in ice-cold 70% ethanol at 4°C for >12h. - After staining cells were incubate 15 minutes instead of 10min.

Publication protocol

After treatment, cells were fixed in ice-cold 70% ethanol at 4°C for >12h, washed twice with PBS and then exposed to PI/RNase A solution for 10min. The percentage of apoptotic cells (sub-G 1 ) and cell cycle distribution were analyzed by flow cytometry.

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Manufacturer protocol

Download the product protocol from BD Biosciences for PI/RNase Staining Buffer below.

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