Cell Cycle and Apoptosis Analysis Kit

Protocol tips

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	- Cells are fixed overnight at 4°C

Protocol tips
- Cells are fixed overnight at 4°C

Publication protocol

Cells were obtained and washed in PBS for two times, followed by permeabilization with cold 70% methanol for overnight at 4°C. Cell cycle for G1, G2, S phase were detected using Cell Cycle and Apoptosis Analysis Kit (Beyotime, Shanghai, China) according to the manufacturer’s instruction and was detected at 488 nm using a flow cytometer (BD Biosciences, San Jose, CA) and analyzed with ModFit Lt (Verity Software House). RNase staining solution (Sigma-Aldrich), incubated for 60 min at room temperature and analyzed using FACSCalibur flow cytometer. The cell cycle distribution was calculated by using Modfit software (Verity software house Inc).


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