Muse® Cell Cycle Assay Kit

Cell cycle assay human - AGS cell line

Experiment
Cell cycle assay human - AGS cell line
Product
Muse® Cell Cycle Assay Kit from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Protocol tips
- After seeding,cells are treated without or with FCP at concentrations of 75 and 150 µg/ml for 24 h at 37°C.

- For or 3 h at −20°C, cells are fixed in cold 70% ethanol (v/v)

Publication protocol

The AGS cells were incubated without or with FCP at concentrations of 75 and 150 µg/ml for 24 h at 37°C, and the cells were collected, washed with cold PBS, and then centrifuged. The pellet was fixed in cold 70% ethanol (v/v) for 3 h at −20°C. The cells were washed once with PBS, and 200 µl was transferred to fresh tube. Muse Cell Cycle kit reagent (200 µl) was added to each tube and incubation was carried out for 30 min at room temperature in the dark. For analysis of apoptosis, the pellet was resuspended in 1 ml media and 100 µl was transferred to a new tube. Then, 100 µl of Muse Annexin V and Dead Cell kit reagent was added to each tube and incubation was carried out for 20 min at room temperature in the dark. Then, stained samples were analyzed using a Muse™ Mini FACS machine (Millipore).

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Papers

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Paper title
Flavonoids isolated from Citrus platymamma induce mitochondrial-dependent apoptosis in AGS cells by modulation of the PI3K/AKT and MAPK pathways.
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Manufacturer protocol

Download the product protocol from Merck Millipore for Muse® Cell Cycle Assay Kit below.

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