INTERFERin®

Protocol tips

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	siRNA concentration - 5 nM Add INTERFERin® to siRNA duplexes and incubate for 10min at RT after homogenization. Incubate the plate at 37°C for 72h.

Protocol tips
siRNA concentration - 5 nM Add INTERFERin® to siRNA duplexes and incubate for 10min at RT after homogenization. Incubate the plate at 37°C for 72h.

Publication protocol

Silencing RNA (siRNA) transfection of melanoma cells was carried out using INTERFERin transfection solution according to the manufacturer’s protocol (Polyplus-transfection, Illkirch, France). Cells were transfected with 5 nM of siRNA (Qiagen) for 72 h before RNA or protein was extracted. As control siRNA, the All-Star negative siRNA sequence (Qiagen) was used, and gene-specific siRNAs targeting siSOX9 (SI00007595, SI00007609) were obtained from Qiagen.

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Manufacturer protocol

Download the product protocol from Polyplus transfections for INTERFERin® below.

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