PI/RNase Staining Buffer

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	- cells were fixed in 70% ethanol at −20°C for 4 h

Protocol tips
- cells were fixed in 70% ethanol at −20°C for 4 h

Publication protocol

Pretreated U2-OS and SAOS2 cells were harvested and washed twice in phosphate-buffered saline (PBS). Then cells were fixed in 70% ethanol at −20°C for 4 h. Cells were washed twice with PBS and stained with propidium iodide (PI)/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA) for 15 min. Cell cycle distribution was then analyzed by flow cytometry with the FACSCalibur flow cytometer (BD Biosciences).

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