Muse® Cell Cycle Assay Kit

Protocol tips

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	- Washed cells with PBS by spinning at 4500 rpm.

Protocol tips
- Washed cells with PBS by spinning at 4500 rpm.

Publication protocol

The proliferation capacity and health ofthe H9c2 cardiomyoblast cells upon contact with MFSA hydro-gel scaffolds were assessed by the determination of DNAcontent using ow cytometry analysis (Muse™Cell Analyzer).The cell culture was carried out as per the above procedure.Then the cell cycle analysis was done by using MUSE cell cyclekit as per the manufacturer's instructions. The kit utilizes apremixed reagent which includes the nuclear DNA intercalatingstains propidium iodide (PI) which discriminates cells atdifferent stages of the cell cycle (G0/G1, S and G2/M) based onthe differential DNA content in each phase. Aer 48 h thescaffolds were removed and the cells were trypzinized andwashed thrice with PBS by spinning at 4500 rpm. The washedcells were then xed overnight using absolute ethanol at20 C. The xed cells were then washed twice with PBS asabove, incubated with cell cycle reagent at room temperature in dark for 30 min, and analysed on Muse ow-cytometer(Millipore, USA)

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Manufacturer protocol

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