Muse® Cell Cycle Assay Kit

Protocol tips

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	- Cells are seeded in DMEM + 10% FBS + 1% PS for 3 days. - Fixed overnight with 70% ethanol at −20 °C

Protocol tips
- Cells are seeded in DMEM + 10% FBS + 1% PS for 3 days. - Fixed overnight with 70% ethanol at −20 °C

Publication protocol

Cell cycle analysis was performed by FACS. Briefly, TTRwt and TTRkd cells cultured in DMEM + 10% FBS + 1% PS for 3 days were trypsinized, collected, and centrifuged (2500 rpm, 5 min), after which the supernatants, containing trypsin, were discarded. Cells were then washed with ice-cold PBS and incubated overnight with 70% ethanol at −20 °C. The ethanol was subsequently removed by centrifugation (300 g for 5 min) and cells were washed with ice-cold PBS. Next, cells were treated with Muse cell cycle reagent (Merck Millipore, Darmstadt, Germany), incubated for 30 min in the dark at room temperature and then analyzed using an EasyCyte5HT unit (Merck Millipore).

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Manufacturer protocol

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