Senescence Detection Kit - Merck

Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer

Experiment
Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer
Product
Senescence Detection Kit - Merck from Merck Millipore
Manufacturer
Merck Millipore

Protocol tips

Upstream tips
Seed 1.5×10^5 cells/well
Protocol tips
Pretreat with 4 µM PAB for 3 days.

Fix the cells with 0.5 ml of Fixative Solution for 10 - 15 min at room temperature

Add 0.5 ml of the Staining Solution Mix to each well. Cover the plate. Incubate overnight at 37°C.

Publication protocol

The MCF-7 cells (1.5×105 cells/well) were seeded into 24-well culture plates (Nalge Nunc International, Penfield, NY, USA). Following incubation overnight, 4 µM PAB was added and the plates were incubated for 3 days. The senescence detection kit was used according to the manufacturer's instructions, as previously described (6). Finally, the cells were observed under a phase contrast microscope (Olympus IX51; Olympus Corporation, Tokyo, Japan) for the development of a blue color.

Full paper   Login or join for free to view the full paper.

Reviews

Senescence Detection Kit - Merck from Merck Millipore has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer using Senescence Detection Kit - Merck from Merck Millipore.

Paper title
Pseudolaric acid B activates autophagy in MCF-7 human breast cancer cells to prevent cell death
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Merck Millipore for Senescence Detection Kit - Merck below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer using Senescence Detection Kit - Merck from Merck Millipore. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms