TaqMan® MicroRNA Reverse Transcription Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Use 1 to 10 ng of total RNA per 15- µL RT reaction	Treat RT mixture for 30 minutes at 16C , 30 minutes at 42C and to inactivate treat the tube at 85C for 5 minutes

Upstream tips
Use 1 to 10 ng of total RNA per 15- µL RT reaction
Protocol tips
Treat RT mixture for 30 minutes at 16C , 30 minutes at 42C and to inactivate treat the tube at 85C for 5 minutes

Publication protocol

Total RNA was extracted from kidney using TRIzol (Invitrogen; Thermo Fisher Scientific, Inc., USA). RT was performed using a TaqMan reverse transcription kit (Applied Biosystems; Thermo Fisher Scientific, Inc.), according to the manufacturer’s protocol. MiRNAs were detected using a TaqMan MicroRNA assay (Applied Biosystems; Thermo Fisher Scientific, Inc.), according to the manufacturer’s protocol. U6 small nuclear RNA was used as an endogenous control. Relative miRNA expression levels were calculated using the 2-ΔΔCq method [24]. The primers of miRNAs are shown in Additional file 1: Table S1.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TaqMan® MicroRNA Reverse Transcription Kit below.

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