Senescence Detection Kit I (histochemical)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Fix the cells with 0.5 ml of Fixative Solution for 10-15 minutes at room temperature. Add 0.5 ml of the Staining Solution Mix. Cover the plate. Incubate overnight at 37°C

Protocol tips
Fix the cells with 0.5 ml of Fixative Solution for 10-15 minutes at room temperature. Add 0.5 ml of the Staining Solution Mix. Cover the plate. Incubate overnight at 37°C

Publication protocol

SA-ß-Gal activity was performed using a Promokine Senescence detection kit (PK-CA577-K320, PromoCell) according to the manufacturer’s instructions. Percentage of SA ß-gal positive cells was counted in 10 randomly selected microscopic fields (magnification ×20; 400–600 cells).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Reporter gene assay β-galactosidase substrates - HUVEC using Senescence Detection Kit I (histochemical) from PromoKine.

Manufacturer protocol

Download the product protocol from PromoKine for Senescence Detection Kit I (histochemical) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Reporter gene assay β-galactosidase substrates - HUVEC using Senescence Detection Kit I (histochemical) from PromoKine. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.