Luminescent β-galactosidase Detection Kit II

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Warm enough Reaction Buffer and Reaction Substrate for the entire experiment to room temperature	Add 200 µl of the Reaction Buffer Mixture to each cell lysate and mix gently. Incubate at room temperature (20–25°C) for 60 min

Upstream tips
Warm enough Reaction Buffer and Reaction Substrate for the entire experiment to room temperature
Protocol tips
Add 200 µl of the Reaction Buffer Mixture to each cell lysate and mix gently. Incubate at room temperature (20–25°C) for 60 min

Publication protocol

U2OS or H1299 cells were transfected with the corresponding reporter (0.4 μg of reporter and 0.2 μg CMV-βgal). Luciferase activity was quantified using the Luciferase Assay System (Promega). Luciferase values were normalized using β-galactosidase activity measured using the Luminescent β-galactosidase Detection Kit II (Clontech Laboratories). Luminescent levels are expressed as a percentage of controls.

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Manufacturer protocol

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