Senescence Cells Histochemical Staining Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Plate 3×104/well.	After 7 days of 100 nM doxorubicin treatement, add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue.

Upstream tips
Plate 3×104/well.
Protocol tips
After 7 days of 100 nM doxorubicin treatement, add 1 ml of the Staining Mixture per well and incubate at 37 °C without CO2 until the cells are stained blue.

Publication protocol

A549 cells (3×104/well) were seeded to 24-well plates for overnight before being treated with 100 nM doxorubicin. After 7 days of the treatment, SA-β-galactosidase staining was carried out using the Senescence histochemical staining kit (Sigma) following the manufacturer's instruction. Cells were incubated at 37 °C for 18 h and photographed.

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Manufacturer protocol

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